Endopeptidase, Granulate

rTrypsin® Granulate

rTrypsin® Granulate (EC is a recombinant product manufactured by microbial fermentation. Its substrate specificity is serine protease and its unit activity is 800 USP/m. Like native trypsin from bovine or porcine pancreas, it's suited to a range of biotechnological processes. Unlike native trypsin, rTrypsin® Granulate is animal component-free. That allows it to meet the stringent safety regulations in place for biologicals and biopharmaceuticals.

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Key Benefits Description
  • Free of animal enzymes

    rTrypsin® Granulate is manufactured by a recombinant process to ISO 9001:2008 standards. That ensures it's not contaminated with animal enzymes such as chymotrypsin or carboxypeptidase.

  • Free of viral and animal-related contaminants

    The manufacturing process for rTrypsin® Granulate is animal origin-free (AOF). That eliminates the risk of viral and animal-related contaminants in the final product. 

  • Stability unaffected by Ca2+ ions

    The stability of porcine trypsin is dependent on the level of free Ca2+ ions in the buffer. By contrast, the stability of rTrypsin® Granulate is unaffected by free Ca2+ ions. 

  • Highly temperature stable

    The exceptional stability of rTrypsin® Granulate permits easy and convenient storage. It also allows routine handling of the enzyme during cell culture procedures. 

  • Wide pH range

    rTrypsin® Granulate is stable for two hours in a pH range of 5-9 at 37°C. 

  • Consistent supply

    We have production facilities on four continents, warehouses all over the world and a flexible and robust distribution network. All this helps us deliver the consistent supply you need.  

rTrypsin® Granulate cleaves peptide bonds at the carboxyl side of lysine and arginine as native trypsin. That allows simple substitution of rTrypsin® Granulate for porcine or bovine trypsin. rTrypsin® Granulate is used for a range of biotechnological processes. These include: 
• Cell dissociation and propagation of multiple cell lines including stem cells
• Vaccine production and processing
• Dissociation of dissected cells prior to cell fixing and sorting
• Cleavage of proinsulin to yield intact insulin